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    mcherry激發波長是多少?

    作者:時間:2019-09-05 10:26瀏覽2105 次

    信息摘要:

    mCherry是一種來自于蘑菇珊瑚(mushroom coral)的紅色熒光蛋白,常有于標記和示蹤某些分子和細胞組分。相對于其他熒光,mCherry的好處在于它的顏色和應用最多的綠色熒光蛋白(GFP)能進行共同標記,并且mCherry相對于其他單體熒光蛋白來說也具有卓越的光穩定型。

    mcherry激發波長是多少?

    mCherry是一種來自于蘑菇珊瑚(mushroom coral)的紅色熒光蛋白,常有于標記和示蹤某些分子和細胞組分。相對于其他熒光,mcherry的好處在于它的顏色和應用最多的GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白(GFP)能進行共同標記,并且mcherry相對于其他單體熒光蛋白來說也具有卓越的光穩定型。

    mcherry紅色熒光蛋白
    吸收和發射波長:

    mcherry的更大吸收/發射峰分別位于587nm和610nm,對光致漂白耐受,熒光非常穩定。

    mcherry紅色熒光蛋白

    常見應用:
    ?mCherry常用于與目的基因組成融合蛋白以及通過IRES或2A與感興趣的蛋白共表達;
    ?啟動子活性研究;
    ?熒光共振能量轉移(fluorescence resonance energy transfer,FRET)和其他定量實驗;
    ?標記細胞或者分子,進行示蹤實驗;

    mcherry紅色熒光蛋白

     mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

     
    m前綴的意義:
    mCherry的m為monmer單體的縮寫,表示mCherry熒光蛋白的形式為單體,這在很多實驗設計中非常重要,比如與目的標記基因組成融合蛋白時。

    成熟時間:
    mCherry具有較快的成熟速度,t0.5為15分鐘,這在一些需要做出快速反應的實驗非常重要。比如啟動子活性報告系統等。

    如何觀察和篩選mcherry在植物中的表達?

    你可以選用LUYOR-3415RG和LUYOR-3260GR便攜式熒光蛋白激發光源來直接觀察和篩選mcherry在植物中有沒有表達。如需進一步了解,請撥打電話153-1756-5658進行咨詢(或直接添加微信15317565658咨詢)。

    mcherry紅色熒光蛋白

    mcherry紅色熒光蛋白的激發波長和發射波長


    實驗室常見熒光蛋白的激發波長和發射波長


    紫外熒光蛋白UV Proteins

    ProteinExcitation Wavelength  Emission Wavelength       
    Sirius355424
    Sandercyanin375630
    shBFP-N158S/L173I375458

     

     

     


    藍色熒光蛋白Blue Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Azurite383450
    EBFP2383448
    mKalama1385456
    mTagBFP2                       399454
    TagBFP402457
    shBFP401458

    青色熒光蛋白Cyan Proteins

    ProteinExcitation WavelengthEmission Wavelength
    ECFP433475
    Cerulean433475
    mCerulean3433475
    SCFP3A433474
    CyPet435477
    mTurquoise434474
    mTurquoise2434474
    TagCFP458480
    mTFP1462492
    monomeric Midoriishi-Cyan470496
    Aquamarine430474

    綠色熒光蛋白Green Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TurboGFP482502
    TagGFP2483506
    mUKG483499
    Superfolder GFP485510
    Emerald487509
    EGFP488507
    Monomeric Azami Green   492505
    mWasabi493509
    Clover505515
    mNeonGreen506517
    NowGFP494502
    mClover3506518

    黃色熒光蛋白Yellow Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TagYFP508524
    EYFP513527
    Topaz514527
    Venus515528
    SYFP2515527
    Citrine516529
    Ypet517530
    lanRFP-ΔS83                      521592
    mPapaya1530541
    mCyRFP1528594

    桔色熒光蛋白Orange Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Monomeric Kusabira-Orange548559
    mOrange548562
    mOrange2549565
    mKOκ551563
    mKO2551565

    紅色熒光蛋白Red Proteins

    ProteinExcitation WavelengthEmission Wavelength
    TagRFP555584
    TagRFP-T555584
    RRvT556583
    mRuby558605
    mRuby2559600
    mTangerine                            568585
    mApple568592
    mStrawberry574596
    FusionRed580608
    mCherry587610
    mNectarine558578
    mRuby3558592
    mScarlet569594
    mScarlet-I569593

    遠紅熒光蛋白Far Red Proteins

    ProteinExcitation WavelengthEmission Wavelength
    mKate2588633
    HcRed-Tandem590637
    mPlum590649
    mRaspberry598625
    mNeptune600650
    NirFP605670
    TagRFP657                            611657
    TagRFP675598675
    mCardinal604659
    mStable597633
    mMaroon1609657
    mGarnet2598671

    近紅熒光蛋白Near IR Proteins

    ProteinExcitation WavelengthEmission Wavelength
    iFP1.4684708
    iRFP713 (iRFP)                     690713
    iRFP670643670
    iRFP682663682
    iRFP702673702
    iRFP720702720
    iFP2.0690711
    mIFP683704
    TDsmURFP642670
    miRFP670642670

    Sapphire-type Proteins

    ProteinExcitation WavelengthEmission Wavelength
    Sapphire399511
    T-Sapphire                             399511
    mAmetrine406

    526

    Red Fluorescent Proteins

    Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

    The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

    Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


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