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                  mcherry激發波長是多少?

                  作者:時間:2019-09-05 10:26瀏覽1796 次

                  信息摘要:

                  mCherry是一種來自于蘑菇珊瑚(mushroom coral)的紅色熒光蛋白,常有于標記和示蹤某些分子和細胞組分。相對于其他熒光,mCherry的好處在于它的顏色和應用最多的綠色熒光蛋白(GFP)能進行共同標記,并且mCherry相對于其他單體熒光蛋白來說也具有卓越的光穩定型。

                  mcherry激發波長是多少?

                  mCherry是一種來自于蘑菇珊瑚(mushroom coral)的紅色熒光蛋白,常有于標記和示蹤某些分子和細胞組分。相對于其他熒光,mcherry的好處在于它的顏色和應用最多的GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白(GFP)能進行共同標記,并且mcherry相對于其他單體熒光蛋白來說也具有卓越的光穩定型。

                  mcherry紅色熒光蛋白
                  吸收和發射波長:

                  mcherry的更大吸收/發射峰分別位于587nm和610nm,對光致漂白耐受,熒光非常穩定。

                  mcherry紅色熒光蛋白

                  常見應用:
                  ?mCherry常用于與目的基因組成融合蛋白以及通過IRES或2A與感興趣的蛋白共表達;
                  ?啟動子活性研究;
                  ?熒光共振能量轉移(fluorescence resonance energy transfer,FRET)和其他定量實驗;
                  ?標記細胞或者分子,進行示蹤實驗;

                  mcherry紅色熒光蛋白

                   mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

                   
                  m前綴的意義:
                  mCherry的m為monmer單體的縮寫,表示mCherry熒光蛋白的形式為單體,這在很多實驗設計中非常重要,比如與目的標記基因組成融合蛋白時。

                  成熟時間:
                  mCherry具有較快的成熟速度,t0.5為15分鐘,這在一些需要做出快速反應的實驗非常重要。比如啟動子活性報告系統等。

                  如何觀察和篩選mcherry在植物中的表達?

                  你可以選用LUYOR-3415RG和LUYOR-3260GR便攜式熒光蛋白激發光源來直接觀察和篩選mcherry在植物中有沒有表達。如需進一步了解,請撥打電話153-1756-5658進行咨詢(或直接添加微信15317565658咨詢)。

                  mcherry紅色熒光蛋白

                  mcherry紅色熒光蛋白的激發波長和發射波長


                  實驗室常見熒光蛋白的激發波長和發射波長


                  紫外熒光蛋白UV Proteins

                  ProteinExcitation Wavelength  Emission Wavelength       
                  Sirius355424
                  Sandercyanin375630
                  shBFP-N158S/L173I375458

                   

                   

                   


                  藍色熒光蛋白Blue Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  Azurite383450
                  EBFP2383448
                  mKalama1385456
                  mTagBFP2                       399454
                  TagBFP402457
                  shBFP401458

                  青色熒光蛋白Cyan Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  ECFP433475
                  Cerulean433475
                  mCerulean3433475
                  SCFP3A433474
                  CyPet435477
                  mTurquoise434474
                  mTurquoise2434474
                  TagCFP458480
                  mTFP1462492
                  monomeric Midoriishi-Cyan470496
                  Aquamarine430474

                  綠色熒光蛋白Green Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  TurboGFP482502
                  TagGFP2483506
                  mUKG483499
                  Superfolder GFP485510
                  Emerald487509
                  EGFP488507
                  Monomeric Azami Green   492505
                  mWasabi493509
                  Clover505515
                  mNeonGreen506517
                  NowGFP494502
                  mClover3506518

                  黃色熒光蛋白Yellow Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  TagYFP508524
                  EYFP513527
                  Topaz514527
                  Venus515528
                  SYFP2515527
                  Citrine516529
                  Ypet517530
                  lanRFP-ΔS83                      521592
                  mPapaya1530541
                  mCyRFP1528594

                  桔色熒光蛋白Orange Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  Monomeric Kusabira-Orange548559
                  mOrange548562
                  mOrange2549565
                  mKOκ551563
                  mKO2551565

                  紅色熒光蛋白Red Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  TagRFP555584
                  TagRFP-T555584
                  RRvT556583
                  mRuby558605
                  mRuby2559600
                  mTangerine                            568585
                  mApple568592
                  mStrawberry574596
                  FusionRed580608
                  mCherry587610
                  mNectarine558578
                  mRuby3558592
                  mScarlet569594
                  mScarlet-I569593

                  遠紅熒光蛋白Far Red Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  mKate2588633
                  HcRed-Tandem590637
                  mPlum590649
                  mRaspberry598625
                  mNeptune600650
                  NirFP605670
                  TagRFP657                            611657
                  TagRFP675598675
                  mCardinal604659
                  mStable597633
                  mMaroon1609657
                  mGarnet2598671

                  近紅熒光蛋白Near IR Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  iFP1.4684708
                  iRFP713 (iRFP)                     690713
                  iRFP670643670
                  iRFP682663682
                  iRFP702673702
                  iRFP720702720
                  iFP2.0690711
                  mIFP683704
                  TDsmURFP642670
                  miRFP670642670

                  Sapphire-type Proteins

                  ProteinExcitation WavelengthEmission Wavelength
                  Sapphire399511
                  T-Sapphire                             399511
                  mAmetrine406

                  526

                  Red Fluorescent Proteins

                  Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

                  The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

                  Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


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