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                  yfp激發光波長和YFP的發射波長是多少?

                  作者:時間:2019-09-05 10:33瀏覽2056 次

                  信息摘要:

                  黃色熒光蛋白(Yellow Fluorescent Protein ,YFP)可以看做綠色熒光蛋白的一種突變體,最初來源于維多利亞多管水母( Aequorea victoria)。相對于綠色熒光蛋白,其熒光向紅色光譜偏移,而這主要是由于蛋白203位蘇氨酸變為酪氨酸。其更大激發波長為514 nm,更大發射波長為527 nm。

                  YFP激發波長為510nm,更大發射波長為527 nm

                  黃色熒光蛋白(Yellow Fluorescent Protein ,YFP)可以看做GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白的一種突變體,最初來源于維多利亞多管水母( Aequorea victoria)。相對于綠色熒光蛋白,其熒光向紅色光譜偏移,而這主要是由于蛋白203位蘇氨酸變為酪氨酸。其更大激發波長為514 nm,更大發射波長為527 nm。

                  黃色熒光蛋白

                   

                  黃色熒光蛋白的激發波長

                  黃色熒光蛋白的發射波長

                  美國路陽生產有多款便攜式激發光源,無需熒光顯微鏡就能輕松觀察篩選黃色熒光蛋白表達陽性。LUYOR-3260CY便攜式YFP激發光源能直接分選YFP表達陽性的植株,能夠分選愈傷組織、植株、種子、菌落等。如欲進一步了解便攜式熒光蛋白激發光源,可直接聯系上海路陽生物技術有限公司的銷售客服。

                  黃色熒光蛋白激發光源

                  Yellow Fluorescent Proteins/常用黃色熒光蛋白的激發波長和發射波長

                  YFP種類 激發光波長(nm) 發射光波長(nm)
                  EYFP 514 527
                  Topaz 514 527
                  Venus 515 528
                  mCitrine 516 529
                  YPet 517 530
                  TagYFP 508 524
                  PhiYFP 525 537
                  ZsYellow1 529 539
                  mBanana 540 553

                   

                  Yellow Fluorescent Proteins

                  Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

                  To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

                  YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

                   黃色熒光蛋白的激發波長和發射波長

                  Fluorochrome Excitation Wavelength (Nanometers) Emission Wavelength (Nanometers)
                  Acridine Orange (Bound to DNA) 500 526
                  Alexa Fluor 488 495 519
                  Alexa Fluor 500 503 525
                  Aminofluorescein 497 519
                  Astrazon Orange R 470 540
                  Aurophosphine 450-490 515
                  BCECF (Biscarboxyethylcarboxyfluorescein) 505 530
                  BODIPY 492/515 (Difluoroboradiazaindacene) 490 515
                  BODIPY 493/503 500 506
                  BODIPY 500/510 509 515
                  BODIPY 505/515 502 510
                  BODIPY FL 503 512
                  Calcein 494 517
                  Calcium Green 506 533
                  CellTracker Green CMFDA (Chloromethylfluorescein Diacetate) 492 517
                  CFDA (Carboxyfluorescein Diacetate) 495 520
                  Chinacrine 450-495 515
                  Cl-NERF 514 (High pH) 540 (High pH)
                  CyQuant GR 480 520
                  DCFH (Carboxydichlorofluorescein) 505 535
                  DiBAC4(3) (Bisdibutylbarbituric Acid) 493 516
                  DiO (Dipentyloxacarbocyanine Iodide) 484 502
                  DiOC16(3) 484 501
                  DiOC18(3) 484 501
                  DiOC2(3) 482 497
                  DiOC5(3) 484 500
                  DiOC6(3) 484 511
                  DiOC7(3) 482 504
                  DM-NERF 510 (High pH) 497 (Low pH) 536 (High pH) 527 (Low pH)
                  DTAF (Fluorescein Dichlorotriazine) 494 520
                  FAM (Carboxyfluorescein) 492 518
                  FDA (Fluorescein Diacetate) 495 520
                  FITC (Fluorescein Isothiocyanate) 494 518
                  FlCRhR (Cyclic AMP Fluorosensor) 500 517
                  Fluo-3 480-506 520
                  Fluo-4 494 516
                  Fluo-4FF 494 518
                  Fluo-5F 494 516
                  Fluo-5N 493 518
                  Fluorescein 494 518
                  Fluorescein Sulfonic Acid 476 519
                  FluoroEmerald 495 524
                  FluorX 494 520
                  FluoZin-1 495 517
                  FluoZin-2 494 521
                  FluoZin-3 494 516
                  GFP-RS (Red-Shifted) 498 516
                  LysoTracker Green DND-26 504 511
                  Magnesium Green 506 531
                  MitoFluor Green 490 516
                  MitoTracker Green FM 490 516
                  NeuroTrace 500/525 500 525
                  NeuroTrace 515/535 515 535
                  Newport Green DCF 506 535
                  Newport Green PDX 490 518
                  Nonyl Acridine Orange 495 519
                  Oligreen 498 518
                  Oregon Green 488 496 524
                  Oregon Green 500 503 522
                  Oregon Green 514 511 530
                  PFB-F (Pentafluorobenzoyl Fluorescein) 492 516
                  Phen Green FL 492 517
                  Phen Green SK 507 532
                  Pico Green 502 523
                  PKH67 496 520
                  Rhodamine 110 496 520
                  Rhodamine 123 507 529
                  Rhodamine Green 502 527
                  Sodium Green 506 532
                  SpectrumGreen 1 497 524
                  SpectrumGreen 2 509 538
                  Stachyose Fluorescein 491 516
                  SYBR Gold 495 537
                  SYBR Green I 498 522
                  SYBR Green II 492 513
                  SYTO 11 508 527
                  SYTO 12 499 522
                  SYTO 13 488 509
                  SYTO 16 488 518
                  SYTO 21 494 517
                  SYTO 23 499 520
                  SYTO 24 490 515
                  SYTOX Green 504 523
                  TAMRA (Carboxytetramethylrhodamine) 504 529
                  YFP/EYFP (Enhanced Yellow Fluorescent Protein) 512 529
                  YOYO-1, YO-PRO-1 491 509

                   


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