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    yfp激發光波長和YFP的發射波長是多少?

    作者:時間:2019-09-05 10:33瀏覽2507 次

    信息摘要:

    黃色熒光蛋白(Yellow Fluorescent Protein ,YFP)可以看做綠色熒光蛋白的一種突變體,最初來源于維多利亞多管水母( Aequorea victoria)。相對于綠色熒光蛋白,其熒光向紅色光譜偏移,而這主要是由于蛋白203位蘇氨酸變為酪氨酸。其更大激發波長為514 nm,更大發射波長為527 nm。

    YFP激發波長為510nm,更大發射波長為527 nm

    黃色熒光蛋白(Yellow Fluorescent Protein ,YFP)可以看做GFP.html' target='_blank' title='綠色熒光蛋白' >綠色熒光蛋白的一種突變體,最初來源于維多利亞多管水母( Aequorea victoria)。相對于綠色熒光蛋白,其熒光向紅色光譜偏移,而這主要是由于蛋白203位蘇氨酸變為酪氨酸。其更大激發波長為514 nm,更大發射波長為527 nm。

    黃色熒光蛋白

     

    黃色熒光蛋白的激發波長

    黃色熒光蛋白的發射波長

    美國路陽生產有多款便攜式激發光源,無需熒光顯微鏡就能輕松觀察篩選黃色熒光蛋白表達陽性。LUYOR-3260CY便攜式YFP激發光源能直接分選YFP表達陽性的植株,能夠分選愈傷組織、植株、種子、菌落等。如欲進一步了解便攜式熒光蛋白激發光源,可直接聯系上海路陽生物技術有限公司的銷售客服。

    黃色熒光蛋白激發光源

    Yellow Fluorescent Proteins/常用黃色熒光蛋白的激發波長和發射波長

    YFP種類 激發光波長(nm) 發射光波長(nm)
    EYFP 514 527
    Topaz 514 527
    Venus 515 528
    mCitrine 516 529
    YPet 517 530
    TagYFP 508 524
    PhiYFP 525 537
    ZsYellow1 529 539
    mBanana 540 553

     

    Yellow Fluorescent Proteins

    Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

    To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

    YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

     黃色熒光蛋白的激發波長和發射波長

    Fluorochrome Excitation Wavelength (Nanometers) Emission Wavelength (Nanometers)
    Acridine Orange (Bound to DNA) 500 526
    Alexa Fluor 488 495 519
    Alexa Fluor 500 503 525
    Aminofluorescein 497 519
    Astrazon Orange R 470 540
    Aurophosphine 450-490 515
    BCECF (Biscarboxyethylcarboxyfluorescein) 505 530
    BODIPY 492/515 (Difluoroboradiazaindacene) 490 515
    BODIPY 493/503 500 506
    BODIPY 500/510 509 515
    BODIPY 505/515 502 510
    BODIPY FL 503 512
    Calcein 494 517
    Calcium Green 506 533
    CellTracker Green CMFDA (Chloromethylfluorescein Diacetate) 492 517
    CFDA (Carboxyfluorescein Diacetate) 495 520
    Chinacrine 450-495 515
    Cl-NERF 514 (High pH) 540 (High pH)
    CyQuant GR 480 520
    DCFH (Carboxydichlorofluorescein) 505 535
    DiBAC4(3) (Bisdibutylbarbituric Acid) 493 516
    DiO (Dipentyloxacarbocyanine Iodide) 484 502
    DiOC16(3) 484 501
    DiOC18(3) 484 501
    DiOC2(3) 482 497
    DiOC5(3) 484 500
    DiOC6(3) 484 511
    DiOC7(3) 482 504
    DM-NERF 510 (High pH) 497 (Low pH) 536 (High pH) 527 (Low pH)
    DTAF (Fluorescein Dichlorotriazine) 494 520
    FAM (Carboxyfluorescein) 492 518
    FDA (Fluorescein Diacetate) 495 520
    FITC (Fluorescein Isothiocyanate) 494 518
    FlCRhR (Cyclic AMP Fluorosensor) 500 517
    Fluo-3 480-506 520
    Fluo-4 494 516
    Fluo-4FF 494 518
    Fluo-5F 494 516
    Fluo-5N 493 518
    Fluorescein 494 518
    Fluorescein Sulfonic Acid 476 519
    FluoroEmerald 495 524
    FluorX 494 520
    FluoZin-1 495 517
    FluoZin-2 494 521
    FluoZin-3 494 516
    GFP-RS (Red-Shifted) 498 516
    LysoTracker Green DND-26 504 511
    Magnesium Green 506 531
    MitoFluor Green 490 516
    MitoTracker Green FM 490 516
    NeuroTrace 500/525 500 525
    NeuroTrace 515/535 515 535
    Newport Green DCF 506 535
    Newport Green PDX 490 518
    Nonyl Acridine Orange 495 519
    Oligreen 498 518
    Oregon Green 488 496 524
    Oregon Green 500 503 522
    Oregon Green 514 511 530
    PFB-F (Pentafluorobenzoyl Fluorescein) 492 516
    Phen Green FL 492 517
    Phen Green SK 507 532
    Pico Green 502 523
    PKH67 496 520
    Rhodamine 110 496 520
    Rhodamine 123 507 529
    Rhodamine Green 502 527
    Sodium Green 506 532
    SpectrumGreen 1 497 524
    SpectrumGreen 2 509 538
    Stachyose Fluorescein 491 516
    SYBR Gold 495 537
    SYBR Green I 498 522
    SYBR Green II 492 513
    SYTO 11 508 527
    SYTO 12 499 522
    SYTO 13 488 509
    SYTO 16 488 518
    SYTO 21 494 517
    SYTO 23 499 520
    SYTO 24 490 515
    SYTOX Green 504 523
    TAMRA (Carboxytetramethylrhodamine) 504 529
    YFP/EYFP (Enhanced Yellow Fluorescent Protein) 512 529
    YOYO-1, YO-PRO-1 491 509

     


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